Age-dependent increased expression and activity of inorganic pyrophosphatase in the liver of male mice and its further enhancement with short- and long-term dietary restriction
| dc.contributor.author | Kharbhih, Wanaiborlang Jala | |
| dc.contributor.author | Sharma, Ramesh | |
| dc.date.accessioned | 2013-11-25T19:47:32Z | |
| dc.date.available | 2013-11-25T19:47:32Z | |
| dc.date.issued | 2013-11-25 | |
| dc.description.abstract | Intracellular orthophosphate and inor- ganic pyrophosphate (PPi) are by-products of multiple biosynthetic reactions. PPi hydrolysis by soluble inorganic pyrophosphatase (iPPase) has been consid- ered as an important homeostatic mechanism. We investigated the expression and activities (U/mg protein) of iPPase in the liver of young and old mice subjected to short- and long-term dietary restriction. The expression level of iPPase was ascertained by the Western blot analysis using anti-iPPase and differen- tial polymerase chain reaction using iPPase specific primer. Older mice showed a significant increase in the expression and activity of iPPase as compared to younger ones. Short-term fasting of 24 h increased the expression and activity of iPPase in the liver of both young and old mice which were reversed upon 24 h of re-feeding them. However, both young and old mice on long-term dietary restriction showed a cumulative increase in the expression and activity of iPPase when compared with their age-matched controls. This might be due to accumulative adaptation to refill energy deficiency of long-term dietary restricted mice for ATP generation via oxidative phosphorylation, where fatty acid activation could be driven by elevated iPPase. | en_US |
| dc.document.department | Department of Biochemistry | en_US |
| dc.document.source | Biogerontology | |
| dc.identifier.uri | https://dspace.nehu.ac.in/handle/1/11241 | |
| dc.journal.pagerange | 6 | en_US |
| dc.language.iso | en | en_US |
| dc.subject | Inorganicpyrophosphatase Aging | en_US |
| dc.subject | Dietary restriction | en_US |
| dc.subject | Liver Fasting | en_US |
| dc.submitter.address | North Eastern Hill University, Shillong 793022 | en_US |
| dc.title | Age-dependent increased expression and activity of inorganic pyrophosphatase in the liver of male mice and its further enhancement with short- and long-term dietary restriction | en_US |
| dc.type | Article | en_US |