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|Title:||Attenuation of age-related increase of protein carbonylation in the liver of mice by melatonin and curcumin|
|Abstract:||Protein carbonyls are formed as a consequence of the oxidative modiﬁcation of proteins by reactive oxy- gen species and are commonly used as a marker of protein oxidation in cells and tissues. Melatonin has free radical scavenging ability besides its classical role as a hormonal signaling agent. Curcumin, a phytochemical, has a wide variety of biological actions including anti-inﬂammatory and antioxidative. In the present study, the effects of melatonin and curcumin on age-related carbonyl content of liver in mice were investigated. Young (1 month) and aged (18 month) were administered with melatonin (10 mg/kg body weight) and curcumin (90 mg/kg body weight) in dimethyl sulfoxide intraperitoneally. Livers were excised from each experimental group and processed. The level of protein carbonylation was assessed spectrophotometrically and further conﬁrmed by Western blotting analysis. Protein carbonyls of liver have been found to be signiﬁcantly higher in 18-month-old mice as compared to 1-month-old mice. The carbonyl content in 1- and 18-month-old mice decreases signiﬁcantly upon administrations of melatonin and curcumin. This study thus suggests that the formation of protein carbonyls in the liver of the aging mice can be prevented by the antioxidative effects of melatonin and curcumin, which may provide health beneﬁts in aging animals.|
|Appears in Collections:||Ramesh Sharma|
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